乌头霜霉病传播机制初步研究
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【摘 要】 目的：明確乌头霜霉病病原菌的繁殖方式及传播途径，为乌头霜霉病的生物防治技术提供理论基础。方法：调查不同种源地乌头霜霉病发病情况，并对患病乌头的根、茎、叶进行石蜡切片，在光学显微镜下观察病原菌的繁殖方式;采用扫描电子显微镜观察乌头病叶上病原菌的定殖、生长以及传播的方式。结果：不同种源地的乌头幼苗霜霉病发病情况不同;通过显微观察，在患病乌头的茎和叶中均发现了卵孢子，在种根中未观察到卵孢子;在电子显微镜下观察乌头霜霉病叶，发现孢子囊以萌发芽管的方式，通过叶片气孔或者伤口定殖于植株上，并通过在细胞间产生吸器从寄主吸取营养，萌发孢囊梗，再产生孢子囊。结论：乌头霜霉病发病与种苗是否带菌有关，其传播可能是乌头霜霉卵孢子在种苗内越冬，春天萌发侵染乌头。
【关键词】 乌头;霜霉病;种苗;繁殖方式;传播机制
【中图分类号】R282.2 【文献标志码】 A【文章编号】1007-8517（2019）21-0016-05
Preliminary Study on the Transmission Mechanism of Downy Mildew of Aconitum carmichaeli Debx.
CHEN Maoting1 SHU Jianqin1 LI Wenmin1 HU Qiqi1 WANG Guangzhi1 OU Hong2*
1.Chengdu University of Traditional Chinese Medicine， Chengdu 611137， China;
2.Chengdu University of Traditional Chinese Medicine Clinical Medical College， Chengdu 611137， China
Abstract：Objective To clarify the propagation mode and transmission route of the pathogen of downy mildew of aconitum， and to provide theoretical basis for the prevention and treatment of downy mildew of aconitum. Methods The incidence of downy mildew was investigated in different provenances of aconitum， and paraffin sections were made on the roots， stems and leaves of the diseased aconitum， and the sexual reproduction of pathogenic bacteria was observed under optical microscope. Scanning electron microscopy was used to observe the colonization， growth and spread of pathogenic bacteria on aconitum leaf. Results The inci...
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