青杨CCoAOMT基因的克隆及其生物信息学分析
摘要:以一年生青杨(Populus cathayana Rehd.)嫩茎为试材,利用RT-PCR技术克隆青杨CCoAOMT 基因,并对其进行生物信息学分析｡结果表明,该基因cDNA序列全长为747 bp,是一个编码248个氨基酸的多肽,蛋白分子量为27.67 kD,等电点为5.19,将该基因命名为PcCCoAOMT,其编码的蛋白质为是亲水性蛋白质;氨基酸序列和结构分析表明,CCoAOMT有一个保守区域;系统进化分析表明,PcCCoAOMT与毛果杨的PtCCoAOMT基因具有很高的同源性(87%),该基因功能可能与青杨木质素的生物合成有关｡
关键词:青杨(Populus cathayana Rehd.);CCoAOMT 基因;克隆;生物信息学
中图分类号:S792.113;Q7文献标识码:A文章编号:0439-8114(2014)11-2670-05
Cloning and Bioinformatics Analysis of CCoAOMT Gene in Populus cathayana Rehd.
LIU Hong-mei,HU Shang-lian,LU Xue-qin,CAO Ying,REN Peng
(College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, Sichuan, China)
Abstract: Raw Populus cathayana Rehd. spear of one year was used to clone, the CCoAOMT gene with RT-PCR and its bioinformatics analysis was carried out. The cDNA sequence was 747 bp in length, encoding 248 amino acids. The molecular weight of protein encoded by the CCoAOMT gene was 27.94 kD. Its theoretical PI was 5.19. The CCoAOMT gene was named as PcCCoAOMT. The protein encoded by the CCoAOMT gene was hydrophilic protein. The results of amino acid sequence analysis showed that the protein encoding by PcCCoAOMT contained one conserved domain. Phylogenetic analysis showed that PcCCoAOMT gene had 87% similarity with the corresponding genes PtCCoAOMT in Populus trichocarpa. It may be involued in biosynthesis of poplar lignin.
Key words: Populus cathayana Rehd.;CCoAOMT gene;cloning;bioinformatics
基金项目:四川省'十二五“重点攻关项目(2011YZGG-10)
木质素是植物体中重要的大分子有机物质,仅...
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