TNF—α椎间盘注射对软骨终板β—catenin与Adamts—5表达影响
[摘要] 目的 探討TNF-α椎间盘注射对大鼠软骨终板β-catenin与Adamts-5蛋白表达的影响与意义。 方法 取SPF级12周龄SD大鼠30只,随机分成空白对照组(A组)、假手术组(B组)、TNF-α椎间盘注射组(C组),每组10只(n=10)。C组大鼠在X线透视下穿刺椎间盘并注射TNF-α 25 μL(10 ng/μL),B组大鼠椎间盘穿刺成功后注射生理盐水25 μL,A组大鼠椎间盘不做任何干预。术后4周采用组织病理学观察软骨终板退变情况,并采用Western-Blot检测软骨终板β-catenin与Adamts-5蛋白表达。 结果 HE染色显示C组大鼠椎间盘软骨终板出现明显退行性改变。Western-Blot检测显示C组大鼠椎间盘软骨终板β-catenin与Adamts-5表达均显著上调,与A组及B组相比,差异有统计学意义(P<0.05)。 结论 TNF-α椎间盘注射可诱发椎间盘软骨终板退变,同时上调软骨终板β-catenin及Adamts-5表达,提示TNF-α上调β-catenin及Adamts-5表达在软骨终板退变中可能发挥了重要作用。
[关键词] TNF-α;软骨终板;β-catenin;Adamts-5
[中图分类号] R681.5 [文献标识码] A [文章编号] 1673-9701(2018)17-0024-04
Effect of intervertebral disc injection of TNF-α on the expression of β-catenin and Adamts-5 in cartilage endplate
WEI Pingyang1 XIA Bingjiang1,2 SHEN Qiong1 HU Songfeng1 HOU Mingsheng1
1.Department of Orthopedics, Shaoxing Hospital of TCM, Shaoxing 312000, China; 2.Zhejiang Chinese Medical University, Hangzhou 310053, China
[Abstract] Objective To investigate the effect and significance of intervertebral disc injection of TNF-α on the expression of β-catenin and Adamts-5 protein in rat cartilage endplate. Methods 30 Sprague-Dawley(SD) rats of SPF grade were randomly divided into blank control group(group A), sham operation group(group B) and TNF-α intervertebral disc injection group(group C), with 10 rats in each group. The rats in group C were given intervertebral disc puncture under X-ray and injected with TNF-α of 25 μL(10 ng/μL). Rats in group B were injected ...
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