4种萜类化合物对蝴蝶兰茎腐病菌的抑制作用及机理的初步研究
摘要以从蝴蝶兰茎腐病斑分离的12株革兰氏阴性菌和2株真菌为试验材料，研究香茶菜属植物中2种二萜（leukamenin E和weisiensin B）以及2种三萜（熊果酸和2α羟基熊果酸）的抑菌活性，并对leukamenin E抑制病原菌尖镰孢生长的机理进行初步研究。结果表明，leukamenin E对2株真菌的抑制作用最强，熊果酸和2α羟基熊果酸次之，weisiensin B最弱；800 μmol/L leukamenin E 可破坏尖镰孢菌丝体细胞内微丝结构，并使菌丝细胞膜通透性显著增加，导致菌丝体极性生长受阻。4种萜类对12株细菌的抑制活性显示了明显的选择性和互补特性，leukamenin E对其中5种细菌有抑制作用，而weisiensin B则对其他4种细菌具有抑制活性，weisiensin B还对2种三萜均无抑制效应的细菌也有较强的抑制活性，揭示了香茶菜植物萜类分子结构多样性具有重要的化学生态学意义。
关键词对映贝壳杉烷二萜；乌苏烷型三萜；蝴蝶兰茎腐病；尖镰孢；抑菌活性
中图分类号:S 436.81文献标识码：ADOI：10.3969/j.issn.05291542.2014.03.007Antibacterial activity and related mechanism of four terpenoids on the
pathogen of Phalaenopsis amabilis stem rotDing Lan,He Miao,Wang Baoqiang,Liu Guo’an（College of Life Sciences, Northwest Normal University, Lanzhou 730070, China）AbstractIn this study, twelve Gramnegative bacteria and two fungi from Phalaenopsis amabilis stem rot were tested to evaluate the antibacterial activity of the entkaurane diterpenoids（leukamenin E and weisiensin B）and the triterpenes (ursolic acid and 2αhydroxy ursolic acid). In addition, the antibacterial activity of leukamenin E to Fusarium oxysporum was preliminarily studied. The results showed that the antibacterial activity of leukamenin E was the strongest to the two fungi, while that of weisiensin B was the weakest and the two triterpenes had moderate activity. Leukamenin E at 800 μmol/L could destroy the Factin cytoskeleton of F. oxysporum, and make the cell membrane of F. oxysporum magnify obviously, resulting in hindered ...
|
== 试读已结束，如需继续阅读敬请充值会员 ==
|
|
本站文章均为原创投稿，仅供下载参考，付费用户可查看完整且有格式内容！
(费用标准：38元/2月，98元/2年，微信支付秒开通！) |
| 升级为会员即可查阅全文 。如需要查阅全文，请 免费注册 或 登录会员 |
|
