烟草绿原酸生物合成途径关键酶基因的研究进展
摘要 绿原酸是重要的植物苯丙素类次生代谢产物之一,与植物的食品风味和药用生物活性等密切相关,其生物合成与调控研究一直是科研人员所关注的焦点。本文综述了烟草绿原酸生物合成途径中关键节点苯丙氨酸解氨酶基因、肉桂酸-4-羟化酶基因、对-香豆酸辅酶A连接酶基因、羟基肉桂酰辅酶A:奎尼酸羟基肉桂酰转移酶基因、羟基肉桂酰辅酶A莽草酸/奎尼酸羟基肉桂酰转移酶基因、对-香豆酸3′-羟化酶基因的研究进展,并进一步展望了烟草绿原酸生物合成代谢调控的研究方向,旨在为烟草绿原酸生物合成途径的深入研究和定向调控提供参考。
关键词 烟草;绿原酸;生物合成;关键酶;基因
中图分类号 S572 文献标识码 A 文章编号 1007-5739(2018)13-0005-04
Research Progress on Key Enzyme Genes in Chlorogenic Acid Biosynthesis Pathway
YANG Yin-ju 1,2,3 CHEN Ai-guo 1,2 LIU Guang-liang 1,2 ZHANG Yan 1,2 WANG Shu-sheng 1,2 *
(1 Tobacco Research Institute of Chinese Academy of Agricultural Sciences,Qingdao Shandong 266101; 2 Key Laboratory of Tobacco Biology and Processing,Ministry of Agriculture; 3 Graduate School of Chinese Academy of Agricultural Sciences)
Abstract Chlorogenic acid is one of the important phenylpropanoids formed by plants secondary metabolism,which is closely related to plant food flavor and medical biological activity. Accordingly,the researches on the chlorogenic acid biosynthetic pathway and its regulation have been the focus of researchers.In this paper,considering the biosynthesis pathway of tobacco chlorogenic acid,we described the molecular regulations of the key nodes containing phenylalanine ammonia-lyase gene,cinnamic acid 4-hydroxylase gene,4-coumarate-CoA ligase gene,Hydroxycinnamoyl-CoA:quinate hydroxycinnamoyl transferase gene,hydroxycinnamoyl CoA shikimate/quinate hydroxycinnamoyltransferase gene,and ρ-coumaroylester 3′-hydroxylases gene.Additionally,the future researches on metabolic...
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