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黑龙江省大豆镰孢根腐病菌鉴定及致病力分析

材料写作网    时间: 2020-08-01 04:21:42     阅读:次     手机站

摘要对黑龙江省不同区域采集的大豆根腐病病原种类进行鉴定,结果表明:分离的296个真菌分离物分属于10个种,分别为尖镰孢(Fusarium oxysporum)、茄镰孢(F.solani)、木贼镰孢(F.equiseti )、轮枝镰孢(F.verticillioides)、半裸镰孢(F.semitectum)、大豆拟茎点种腐病菌(Phomopsis longicolla)、瓜果腐霉(Pythium aphanidematum)、茄丝核菌(Rhizoctonia solani)、禾谷镰孢(F.graminearum)、厚垣镰孢(F.chlamydosporum),各分离物分离频率分别为68.24%、7.09%、5.41%、4.05%、3.38%、3.38%、3.04%、2.70%、2.03%和0.68%。利用‘合丰25’测定所有镰孢菌分离物的致病力,病情指数在30以上的分离物占镰孢菌总数的23.42%,其中尖镰孢为18.22%。在中等以上(病情指数>30)致病力镰孢菌分离物中,尖镰孢占77.78%。黑龙江省不同地理区域均有致病力较强的分离物,分布不均匀。大豆花芽分化期分离的致病力较强,尖镰孢出现频率比鼓粒期大。本研究为大豆根腐病的田间防治和抗源筛选的研究奠定了基础。

关键词大豆;根腐病;病原菌;尖镰孢;致病力

中图分类号:S 435.29文献标识码:ADOI:10.3969/j.issn.05291542.2014.03.031Identification and virulence of Fusarium spp. causing

soybean root rot in Heilongjiang ProvinceZhang Li1,2,Geng Xiaobing1,2,Wang Chunling1,2,Li Yonggang1,2(1. Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural

University, Harbin150030, China; 2. Key Laboratory of Physiology, Ecology and Genetic Improvement of Cold

Crops of Higher Education of Heilongjiang Province of China, Harbin150030, China)AbstractThe pathogens causing soybean root rot in Heilongjiang Province were identified. Totally, 296 fungal isolates were isolated from diseased plants collected from Harbin, Jiamus, Heihe, Qiqihaer, and Jixi regions, and these isolates were identified as 10 fungal species, Fusarium oxysporum, F.solani, F.equiseti, F.verticillioides, F.semitectum, Phomopsis longicolla, Pythium aphanidematum, Rhizoctonia solani, F.graminearum, F.chlamydosporum, and the p...



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