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柑橘炭疽病菌的生物学特性及枯草芽胞杆菌对其的抑制作用

材料写作网    时间: 2020-08-01 04:23:17     阅读:次     手机站

摘要:近5年来,湖南麻阳苗族自治县的柑橘种植园发生柑橘炭疽病。采集发病柑橘植株,分离其病原菌,从病原菌的培养性状和形态学特征、内转录区(ITS)序列分析和系统发育关系比较等方面对该病原菌进行了鉴定,并探索了枯草芽胞杆菌(Bacillus subtilis)CGMCC 1.354对其的抑制作用。结果表明:该地区柑橘炭疽病菌为盘长孢状刺盘孢(Colletotrichum gloeosporioides)。枯草芽胞杆菌CGMCC 1.354对该病原菌具有较强的抑制作用,其分泌的抑菌物质能抑制柑橘炭疽病菌菌丝生长和孢子萌发。

关键词:柑橘炭疽病;盘长孢状刺盘孢;枯草芽胞杆菌;抗菌活性

中图分类号:S 436.661.14文献标识码:ADOI:10.3969/j.issn.05291542.2016.01.011Biological characteristics of citrus pathogen Colletotrichum gloeosporioides

and the inhibitory effect of Bacillus subtilis on the fungusZou Juan,Yao Zhuo,Shang Yonghua(Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province,

Department of Life Sciences, Huaihua University, Huaihua418000, China)AbstractAnthracnose on citrus has occurred in Mayang Miao Autonomous County, Hunan Province in the recent five years. The pathogen causing citrus anthracnose was isolated from citrus orchards of Mayang Miao Autonomous County. Based on cultural and morphological characteristics, ribosomal DNA (rDNA) internal transcribed spacer (ITS) sequence analysis and phylogenetic relationships with other species of Colletotrichum, the pathogen was identified as Colletotrichum gloeosporioides. Bacillus subtilis CGMCC 1.354 showed strong antagonistic activity against the pathogen, and the antifungal substance produced by B.subtilis CGMCC 1.354 strongly inhibited the hyphal growth and spore germination of C.gloeosporioides.

Key wordscitrus anthracnose;Colletotrichum gloeosporioides;Bacillus subtilis;antagonistic activity 湖南省麻阳苗族自治县是该省柑橘的主产县,柑橘种植面积近1.3万hm2,年...



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