扬州地区鸡病原性大肠杆菌的分离与鉴定
摘要对扬州市大型鸡场的大肠杆菌病情况进行了流行病学调查和临床诊断,对病死鸡进行了病理剖检,分离出病原菌142株,经生化试验、血清学鉴定,确诊为病原性大肠杆菌的128株。对扬州市大肠杆菌病常见血清型的种类、比例、分布有所了解,同时通过相关的致病性试验和药敏试验,为临床用药和免疫计划提供了可参考的理论依据。
关键词鸡;大肠杆菌;分离;鉴定;江苏扬州
中图分类号 S858.31 文献标识码A文章编号 1007-5739(2009)15-0306-02
AetiologicalIsolationandIdentificationofE.colifromChickeninYangzhouArea
WU Hua-jun 1REN Wen 2ZHOU Mei-xia 2ZHANG Chun-yun 3 *
(1 Yangzhou Livestock Farm Veterinary Station,Yangzhou Jiangsu 225009; 2 Veterinary Station ofBali Town ,Development Zone of Yangzhou City; 3 Bureau ofAgriculture and Forestry ofYizheng City)
AbstractThe investigation on epidemiology and clinic diagnosis of E.coli were carried out in large chicken houses of Yangzhou in 2006. A total of 142 isolations were obtained from binganatomizing infected and dead chicken, and 128 isolations were identified through biochemical reactions and serotype test. The kinds of E.coli serotypes as well as the percent and distribution in Yanngzhou was investigated, and it might had potential effect onclinical drug-use and immunity plan through pathogenicity test and sensitive experiment.
Key wordschicken;E.coli;isolation;identification; Yangzhou in Jiangsu
鸡大肠杆菌病是由致病性大肠杆菌引起的多种疾病的总称,是家禽最常见的病原菌之一。主要存在于病鸡的肠道及分泌物与其他组织器官中,病鸡、鸡粪及污染种蛋为主要传染源。在生产中病鸡死鸡处理不当、种蛋消毒不严格、鸡舍及四周环境消毒不彻底、饲养管理差、饲喂污染的饲料和水等,均可促进该病的流行。该病全年均可发生,但以冬季和夏季多发,常呈地方性流行。鸡大肠杆菌病最常见的血清型是O1、O2、O78和O35,生产中使用的疫苗也以这几种为主。但从国内众多报道看,我国分离到的禽源性大肠杆菌的血清型众多,且不同地区差异较大,这将制约疫苗的免疫效果。
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