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红掌细菌性疫病病原菌遗传多样性的RAPD和Rep—PCR分析

材料写作网    时间: 2020-08-12 05:00:42     阅读:

摘 要 由地毯草黄单胞菌花叶万年青致病变种引起的细菌性疫病是红掌生产中最具破坏性的病害。本研究对来自全国8个省市主产区的93个红掌细菌性疫病菌株,采用RAPD和Rep-PCR(ERIC、BOX、REP)分子标记进行了遗传多样性分析。筛选出的13个RAPD引物共扩增出131个清晰、重复性好的条带,而Rep-PCR共扩增出65个条带,所有位点多态性比率为100%。基于RAPD和Rep-PCR结果计算的群体遗传距离显著相关,综合2种标记类型的分型结果进行UPGMA聚类分析,表明我国红掌细菌性疫病具有高度的遗传多样性,在遗传相似性系数范围0.519 1~0.984 7,在0.63水平时可以将所有菌株划分为3个组群,分别包括58、28、7个个体,病菌群体的遗传多样性与其寄主品种、地理来源没有明显相关性。本研究为红掌细菌性疫病监测和抗病育种提供了科学依据。

关键词 红掌;细菌性疫病;遗传多样性;RAPD;Rep-PCR

中图分类号 S436.8 文献标识码 A

Abstract Bacterial blight is the most devastating disease of anthurium caused by Xanthomonas axonopodis pv. dieffenbachiae (Xad). In this study, ninety-three isolates of Xad from different geographical locations in China were analyzed for genetic diversity using RAPD and Rep-PCR (ERIC, BOX, REP). A total of 131 clear and reproducible bands were amplified from 13 selected RAPD primers, and 65 bands were amplified by Rep-PCR. The polymorphism rate of all sites was 100%. Based on the fingerprint data, cluster analysis were performed by using the unweighted pair group method, arithmetic average (UPGMA) program, which showed a high genetical diversity among the isolates, with genetic similarity varied from 0.519 1 to 0.984 7. At a similarity of 0.63, all isolates were grouped into three genetic groups (Ⅰ-Ⅲ), including 58, 28, 7 isolates, respectively. Moreover, no significant correlation between genetic diversity and geographical origin of the isolates. This present data would provide a scientific support for blight monitoring ...

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