杀松材线虫海洋细菌HT11的鉴定及培养条件研究
摘要[目的] 从海洋中分离并筛选具有杀松材线虫活性的细菌。[方法] 采用稀释涂布平板法对采自青岛近海海域的样品进行细菌分离，采用浸渍法测定菌株的杀线虫活性，并对筛选菌株的形态学、生理生化特性和16S rDNA序列进行测定，通过单因素试验筛选产生杀线虫活性物质的最适培养条件。[结果] 共分离到36株海洋细菌，根据杀线虫活性筛选出1株具有高杀线虫活性的细菌HT11。该菌株分离自牡蛎，其培养滤液处理松材线虫24 h的校正死亡率高达92.41%。根据形态学、生理生化特性和16S rDNA序列测定及其系统发育分析结果，将菌株HT11鉴定为考克氏菌属（Kocuria）。单因素试验结果表明，该菌株在营养肉汤（NB）培养基中产生杀线虫活性物质的最适培养条件为：海水浓度100%，初始pH 7.0，培养温度25 ℃，培养时间3 d。[结论] 该研究可为海洋微生物资源的开发及其杀松材线虫天然活性物质的利用提供理论依据。
关键词海洋细菌；杀线虫活性；松材线虫；鉴定；培养条件
中图分类号S767.3+2文献标识码A文章编号0517-6611（2016）04-024-05
Identification and Culture Conditions of Marine Bacterium HT11 with Nematicidal Activity against Bursaphelenchus xylophilus
CHEN Congcong，GUO Qunqun，WANG Chao，GUO Daosen* et al （College of Life Sciences，Qingdao University，Qingdao，Shandong 266071）
Abstract [Objective] To isolate and screen the marine bacteria with nematicidal activity against Bursaphelenchus xylophilus from the sea.[Method] The marine bacterial strains were isolated from the samples collected from the subtidal zones near Qingdao coast using the dilution and streak plate method； nematicidal activity of strains were detected by immersion method.The morphology，physiological and biochemical characters，and 16S rDNA sequence of the screened strains were also detected.The optimal cultivation conditions to produce nematicidal activity substances were detected by single factor test.[Result] A total of 36 marine bacterial strains were isolated from the samples.One of these strains HT11 had high n...
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