进境百合种球中镰刀菌的分离鉴定
摘要 [目的]从日本进境百合种球中发现带有褐色、不规则病斑的种球，对其进行病原菌分离培养，以明确其病原种类及分类地位。[方法]通过菌落形态特征观察、ITS序列分析、致病性测定对该分离物进行鉴定。[结果]该分离物接种百合种苗引起枯萎反应，ITS序列和层出镰刀菌（Fusarium proliferatum）的序列相似性为100%，结合分离物的菌落特征和孢子特征将病原菌鉴定为层出镰刀菌。[结论]引起百合种球病斑的病原菌为层出镰刀菌。
关键词 百合；种球；层出镰刀菌；鉴定
中图分类号 S41；S436.8 文献标识码 A 文章编号 0517-6611（2017）04-0008-03
Identification of Fusarium proliferatum in Introduced Lily Bulbs
LI Min1， SONG Yun-zhe2， YU Yun1， SHEN Jian-guo1* et al
（1.Inspection and Quarantine Technology Center， Fujian Entry-Exit Inspection and Quarantine Bureau， Fuzhou， Fujian 350000；2.College of Plant Protection， Fujian Agriculture and Forest University， Fuzhou， Fujian 350002）
Abstract [Objective] The pathogene was isolated and cultured from the lily bulbs introduced from Japan which had brown and irregular disease spot， so as to determine its taxonomic status. [Method] The pathogene were identified by pathogenicity determination，morphology observation and ITS sequence analysis. [Result] The ITS sequence of the pathogen causing wilt in lily identity of 100% with Fusarium proliferatum published in GenBank. [Conclusion] The results indicated that the pathogene isolated from the lily bulbs with black brown and irregular disease spot were F. proliferatum.
Key words Lily；Bulbs；F. proliferatum；Identify
鐮刀菌可引起百合枯萎病，是影响百合生产的重要病害之一[1]。该病原菌主要侵染百合肉质根或鳞茎盘基部，引起肉质根和盘基变褐腐烂，并可向上发展导致鳞片出现褐色凹陷病斑，后期鳞片从盘基散开并剥落。罹病鳞茎的植株明显矮化，叶片由上而下黄化，最后枯萎而死[2]。2016年福建出入境检验检疫局从日本进境的百合种球中发现带有褐色、不规则病斑的种...
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