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ADMA对单核细胞来源的树突状细胞成熟和免疫的影响

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中图分类号:R446 文献标 识码:A 文章编号:1009_816X(2010)06_0428_04

DOI:10.3969/j.issn.1009_816X.2010.06.08 Effect of Asymmetrical Dimethylarginine(ADMA)on Maturation and Function of Den dritic Cells Drived from Monocyte.XU Hua_bao, ZHU Wei_guo, LI Shan, et al. Zhuji People"s Hospital, Zhejiang 31180 0,China

[Abstract] ObjectiveThe purpose of this study is to explore the mechanism of ge neration and progress of AS by investigating the effect of ADMA on the expressio n of dendritic cells(DCs) and the interaction between DCs and T lymphocytes.MethodsImmunophenotype, such as HLA_DR, CD1a, CD86 and CD83, of ADMA_treated DCswas monitored by flow cytometry. Allogeneic T cell activation by ADMA_treated DC s was tested by mixed lymphocyte reaction (MLR). The apoptosis of ADMA_treated D Cs was measured by flow cytometry using a Annexin V_FITC. The expression of IL_ 10, IL_12 and TNF_α on ADMA_treated DCs was detected by immunofluorescence sta in.Results The majority of immature DCs cultured in control cultures with GM_C SF and IL_4 expressed high level of HLA_DR, moderate levels of CD1a and CD86, a n d low level of CD83, while the mature DCs exposed to LPS expressed high levels o f HLA_DR, CD86 and CD83, moderate level of CD1a. The presence of ADMA did not c hange the phenotype of immature DCs and high concentration ADMA inhibit the matu ration of DCs. Immature DCs in high concentration ADMA were less effective in st imulating T_cell proliferation. And immature DCs in high concentration ADMA weremore effective to apoptosis. The expression of ...

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