山东省黄瓜细菌性茎软腐病病原菌鉴定与抗病品种筛选
摘要：本试验以黄瓜细菌性茎软腐病病原菌为研究对象，对黄瓜田间发病情况进行观测，分离病原菌并对其形态、致病性及生理生化特性进行分析测定和分子生物学鉴定，同时利用该菌对36份黄瓜资源进行抗病筛选。结果表明：研究地块黄瓜细菌性茎软腐病发病率为5.62%～43.76%，平均为24.69%。病田中分离得到的黄瓜细菌性茎软腐病病原菌经鉴定为荧光假单胞菌（Pseudomonas fluorescens）。36份黄瓜资源中筛选得到2份高感资源，24份感病资源，10份中抗病资源。
关键词：黄瓜细菌性茎软腐病；荧光假单胞菌；抗病资源
中图分类号：S436.421.1文献标识号：A文章编号：1001-4942（2018）07-0133-05
Abstract In this experiment， with the pathogen of cucumber bacterial stem soft rot as study object， the morbidity situation of cucumber in the field was observed. The morphological， pathogenicity， physiological and biochemical characteristics of the pathogenic bacteria were analyzed and molecular biology identification was conducted. Then the bacteria was used to screen the resistant varieties from 36 cucumber resources. The results showed that the incidence of cucumber bacterial stem soft rot was 5.62%～43.76%， with an average of 24.69%. The pathogen of cucumber bacterial stem soft rot isolated from the disease field was identified as Pseudomonas fluorescens. There were 2 high-susceptive cultivars， 24 middle-susceptive cultivars and 10 resistant cultivars in 36 cucumber resources.
Keywords Cucumber bacterial stem soft rot； Pseudomonas fluorescens； Resistant resources
黄瓜为葫芦科黄瓜属一年生草本植物，富含维生素、蛋白质、尼克酸、钙、磷、铁等营养成分，具有清热止渴、利水消肿的作用[1]，是我国一种日常食用的蔬菜。
黄瓜细菌性茎软腐病是黄瓜上常见且严重发生的病害之一，主要为害叶片、叶柄，还可侵染茎蔓和果实，因受害部位会流出胶状物，又叫'黄瓜流胶病“。该病近几年在山东省、山西省、河北省、河南省、辽宁省等黄瓜主产区大面积发生[2]，造成严重经济损失。叶片感病多从叶缘开始发病，形...
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