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7个地理种群小鼠TLR9基因的遗传多样性分析

材料写作网    时间: 2020-09-22 04:05:52     阅读:


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摘要 [目的]分析我国7个地理种群小鼠TLR9基因的遗传多样性。[方法]利用PCR技术扩增并测序7个地理种群100个小鼠样品的TLR9基因。使用MEGA 7.0、DnaSP等软件对基因序列进行分析,计算遗传距离、遗传多样性,并构建系统发育树。[结果]获得的TLR9基因片段长度为1 030 bp,位于参与对病原体相关分子模式识别的LRR结构域的编码区,A、T、C、G的平均含量分别为22.9%、22.2%、32.8%和22.0%,各地理种群间碱基含量没有明显差异。通过计算各地理种群间遗传距离及核苷酸多样性发现,这7个地理种群小鼠TLR9基因序列较为保守,并无明显遗传分化。以褐家鼠(NM_198131) 作为外群构建系统发育树,其结果与遗传多样性分析相一致。[结论]7个地理种群小鼠TLR9基因遗传多样性较低,具有极高的保守性。

关键词 小鼠;TLR9 基因;遗传多样性

中图分类号 Q953文献标识码 A

文章编号 0517-6611(2019)10-0089-03

Abstract [Objective]To analyze the genetic diversity of TLR9 gene in Mus musculus from 7 geographical populations of China.[Method] PCR technology was used to amplify and sequence TLR9 gene of 100 mice samples in 7 geographical populations.Using MEGA 7.0,DnaSP and other software,the sequences of TLR9 gene were analyzed,the genetic distance and genetic diversity were calculated,and the phylogenetic tree was constructed.[Result]The length of TLR9 gene fragment was 1 030 bp.TLR9 gene was located in the encoding region of LRR domain, which was involved in the identification of pathogeelated molecular patterns.The average content of bases A,T,C,G were 22.9%,22.2%,32.8% and 22.0% respectively.There was no significant difference in base composition among different geographical populations.Through calculating the genetic distance and nucleotide diversity among the geographical populations,it was found t...

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