广东不同地区柑橘黄龙病菌的遗传多样性分析
摘要不同基因型的黄龙病菌可能具有不同的致病模式，为了探明广东省不同基因型黄龙病菌的分布特点，为该病害的流行学研究提供重要依据，本研究利用PCRRFLP的方法研究了广东省柑橘产区7个市县黄龙病菌外膜蛋白(outer membrane protein, OMP)基因的遗传多样性，并对各地黄龙病菌的omp基因进行克隆测序，构建了系统发育树。采用4种限制性内切酶消化各地区的柑橘黄龙病菌omp基因分别产生了不同的RFLP指纹图谱，每种限制性内切酶均产生了2种类型的酶切谱带；系统进化分析结果显示：广东地区的柑橘黄龙病菌属于韧皮部杆菌亚洲种，且亲缘关系非常近。广东地区黄龙病菌在omp基因水平上未见显著差异，未发现其他优势种类。
关键词柑橘黄龙病菌；omp；RFLP；遗传多样性
中图分类号:S 436.66；S 432.4文献标识码：ADOI：10.3969/j.issn.05291542.2014.01.010Genetic diversity of Candidatus Liberibacter isolates from different
geographical regions of Guangdong ProvinceHuang Yonghui,Cheng Baoping,Peng Aitian,Song Xiaobing,Ling Jinfeng,Chen Xia(Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangdong
Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou510640, China)AbstractHuanglongbing (HLB) is a destructive disease on citrus, and pathogens with different genotypes may possess different pathogenicity. Ascertain the distribution of Candidatus Liberibacter asiaticus with different genotypes in Guangdong Province could provide an important basis for the studies of disease epidemics. In this study, PCRRFLP analysis based on the outer membrane protein gene (omp) was conducted with disease samples from different regions. These omp sequences were further sequenced and phylogenetic tree was constructed. The amplified omp genes of Ca. Liberibacter asiaticus were digested with four different restrictive endonucleases and several different gene polymorphisms were produced. Each restrictive endonuclease generated two types of ...
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