紫外氯化锂复合诱变哈茨木霉产生多菌灵抗药性菌株的研究
摘要
多菌灵是植物病害防治中常用的化学杀菌剂，野生型哈茨木霉对多菌灵比较敏感。为了能够更好地将哈茨木霉菌应用于实践，本试验采用紫外线氯化锂复合诱变的方法，实现哈茨木霉菌株对多菌灵的抗性改良。试验共获得315株正向突变菌株，其中hcb35菌株抗性能力较强。利用毒力测定方法检测了多菌灵对hcb35有效抑菌中浓度，及hcb35对多菌灵的抗药遗传稳定性；并利用对峙试验及显微观察检测其抑菌能力。结果显示，与哈茨木霉出发菌株hc相比，多菌灵对哈茨木霉突变株hcb35菌株的有效抑菌中浓度提升285%；连续转接12代后，hcb35菌株抗药性相对稳定且抑菌效果较出发菌株无明显差异，表明应用紫外氯化锂复合诱变哈茨木霉可以获得遗传稳定的耐多菌灵突变株。
关键词
复合诱变；哈茨木霉；多菌灵；抗药性；抑菌效果
中图分类号：
S 476
文献标识码：A
DOI：10.3969/j.issn.05291542.2015.05.013
Abstract
Carbendazim is one of commonly used chemical fungicides in plant disease control， and wild type Trichoderma harzianum is very sensitive to carbendazim. In order to make better use of T. harzianum strains， the improvement of carbendazimresistance in T. harzianum strains was studied by UVLiCl composite mutagenesis. A total of 315 positive mutation strains were obtained， among which the hcb35 strain was better than others. The 50% effective inhibition concentration （EC50） and genetic stability of carbendazimresistance was detected， and the inhibition efficacy of T. harzianum was tested by the method of confrontation and microscopic observation. The results showed that， compared with the T. harzianum starting strain hc， the EC50of carbendazim against hcb35 strain was increased by 285%. The carbendazimresistance of hcb35 strain was relatively stable and the inhibition to the target pathogen had no significant difference after subculture for 12 generations. It suggested that the carbendazimresistant strain of T. harzianum mutated b...
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