椒蒿精油对几种植物病原菌的抑制作用研究
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摘 要：目的：研究椒蒿精油对植物病原真菌的抑制作用，探索其在抑菌方面的功能，为天然抗菌剂的开发提供理論依据。方法：以椒蒿精油为原料，采用菌丝生长速率法，测定其对5种植物病原真菌的抑菌作用。结果：浓度为5.0μL/mL的椒蒿精油处理5d，对灰葡萄孢菌有100%的抑制作用。最低抑菌浓度（MIC）测定显示，椒蒿精油对5种供试菌的抑制作用从强到弱依次为灰葡萄孢菌>油菜菌核病菌>辣椒疫霉病菌>棉花枯萎病菌>菜豆炭疽病菌。结论：椒蒿精油对植物病原真菌具有良好的抑制作用，在农药领域中，用椒蒿精油防治植物病原真菌具有很好的应用潜力。
关键词：椒蒿精油;植物病原真菌;菌丝生长;抑菌作用
中图分类号 R284 文献标识码 A 文章编号 1007-7731（2019）16-0085-05
Abstract：To study the inhibitory effect of Artemisia essential oil on plant pathogenic fungi and explore its antibacterial function，so as to provide a theoretical basis for the development of natural antimicrobial agents. The antibacterial activity of Artemisia essential oil was study by the method of mycelial growth rate，which was used to 5 plant pathogenic fungi. The treatment of 5d with Artemisia essential oil，a volume fraction of 5.0μL/mL showed a 100% inhibitory effect on Botrytis cinerea. The determination of minimum inhibitory concentration （MIC） showed that the inhibition effect of Artemisia essential oil on 5 kinds of tested bacteria from strong to weak were，Brassica sclerotiorum、Sclerotinia sclerotiorum、 Phytophthora capsici，Fusariurn oxysporum f、Colletotrichum linde-muthianum. The results showed that the essential oil of Artemisia had a good inhibitory effect on plant pathogenic fungi，and it had good application potential in controlling plant pathogenic fungi with Artemisia essential oil i...
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