40种中药材提取物对烟草黑胫病菌的抑制作用
摘要：测定了崂山40种常见中药材提取液对烟草黑胫病菌的抑菌活性。结果表明，10 g/L乙醇提取物浓度下，有11种植物（占27.5%）提取物对烟草黑胫病菌抑制率达100%，有28种（占70%）抑菌率达80%以上。对公丁香提取液相对含量较高的4种组分的抑菌效果进行了进一步测定，结果表明，丁香酚和乙酸丁香酚酯10倍稀释液对黑胫病菌抑制率高，分别为100%和60.19%，说明丁香酚和乙酸丁香酚酯为公丁香的主要抑菌活性成分。本研究表明，公丁香提取物中活性成分丁香酚具有防治烟草黑胫病的良好应用潜力。
关键词：中药材；Phytophthora nicotiana；公丁香；抑菌活性
中图分类号：S435.72文章编号：1007-5119（2017）01-0064-04DOI：10.13496/j.issn.1007-5119.2017.01.011
Abstract： In this study， antifungal activities of ethanol extracts of 40 species of Chinese herbal medicines against P. nicotianain vitro were determined. The results showed that antifungal plant resources in LaoshanMountain were rich. The inhibitive rates of eleven species （27.5%） reached 100% at the concentration of 10 g/L. 28 species had good inhibitive effects over 80% against P. nicotiana. Determination of the antifungal activities of four fractions with relatively high content in the extracts of clove showed that 10-fold dilution of eugenol possesses the highest inhibitive rate （100%） against P. nicotiana， while the inhibitive rate of eugenyl acetate was 60.19%. This study suggests that a big variety of plant species in LaoshanMountain have antifungal activities， and the active ingredient eugenol of clove ...
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