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小麦腐霉根腐病原鉴定Ⅰ

材料写作网    时间: 2020-08-01 04:23:22     阅读:次     手机站

摘要:2015年冬小麦返青期,山东省青岛市、临沂市的冬小麦出现缺苗断垄现象,叶片出现萎蔫症状,植株矮小,生長不良,毛细根上出现似开水烫过样水渍状病斑,重者小麦根系及茎基部变褐。本研究对其病原菌进行分离培养、致病性测定、形态学观察及分子生物学鉴定。结果表明,引起两地冬小麦根腐病(称为腐霉根腐病)的病原菌为刺腐霉(Pythium spinosum),该病原菌菌落白色,菌丝发达,分枝繁茂。该研究结果可为冬小麦腐霉根腐病防治提供科学依据。

关键词:腐霉根腐病; 刺腐霉; 冬小麦

中图分类号:S435.121.4+7 文献标识号:A 文章编号:1001-4942(2017)08-0104-04

Abstract A disease was observed on winter wheat in Qingdao and Linyi regions of Shandong Province in 2015. The infected wheat showed wilt leaves, water-soaked roots and dwarf plants, and the seriously infected ones had browned roots and stem bases. Two Pythium-like strains were isolated from the infected wheat roots. Based on their morphology biological characteristics, pathogenicity and ITS sequences, the two strains were all identified as P. spinosum. The colonies were white on CMA, and mycelia developed well. The research results could provide scientific bases for prevention and control of wheat pythium root rot.

Keywords Pythium root rot; P. spinosum; Winter wheat

由腐霉菌引起的小麦根腐病(小麦腐霉根腐病)是世界性病害,现全国均有分布。小麦受到腐霉菌Pythium spp.侵染后,根系常表现为变褐腐烂,地上部分生长不良,产量降低,种子和幼苗在出土前就可霉烂和死亡。我国东北、西北春麦区发生重,黄淮海冬麦区也发生普遍。近年来,山东青岛和临沂莒南地区返青后冬小麦叶片变黄,呈点片发生,发病轻的植株根尖水渍状,似开水烫过,地上部分生长不良。本研究通过病原菌分离、致病性测定、形态学观察并结合腐霉菌株rDNA-ITS序列比对结果,明确引起该地区冬小麦根腐病(称其为小麦腐霉根腐病)的病原菌,以期为该病害发生规律的探究和综合防治措施的制定提供理论依据。

1 材料与方法

1.1 病害调查与症状观察

2014年2”3月在山东省青岛...



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