柞蚕核型多角体病毒基因组编码的VmiRNA筛选及功能分析
摘要 [目的]VmiRNA不仅可以控制自身基因，还可调节宿主的稳定表达，研究VmiRNA的功能有助于从分子水平说明核型多角体病毒与柞蚕的寄生关系，从而改进对病毒的防控措施。[方法]利用生物信息学及实时定量PCR等分子生物学相关技术，对柞蚕核型多角体病毒编码的miRNA及其作用的靶标基因进行研究。[结果]经预测和筛选得到了ApNPV编码的miRNA MD217，与其有关的宿主靶基因14种，主要参与细胞组成、生化过程以及分子功能等途径，涉及到生物体细胞内一些催化、免疫反应等过程；实时定量PCR结果表明，MD217在病毒感染后上调表达，脂肪体组织内的靶基因MAPKK7表达水平与MD217的表达呈正相关。[结论]miRNA MD217在病毒感染后诱导表达，推测很可能降低了宿主免疫系统的防御能力，并同时影响靶基因MAPKK7的功能表达，从而避免了在免疫应答过程中的免疫清除。
关键词 柞蚕；核型多角体病毒；miRNA；靶标基因
中图分类号 S188 文献标识码 A 文章编号 0517-6611（2018）23-0065-04
Abstract [Objective] VmiRNA can not only control its own gene，but also regulate the stable expression of the host.Studying the functions of VmiRNA can help to explain the parasitic relation between nuclear polyhedrosis virus and Antheraea pernyi on molecular level，thus improving preventions and controls of the virus.[Method] In this study，molecular biological techniques such as bioinformatics and Quantitative Realtime PCR were employed to research the miRNA of Antheraea pernyi nuclear polyhedrosis virus and its target gene.[Result] The miRNA MD217，which was encoded by ApNPV，was obtained by predicting and screening.The analysis showed that there were 14 host target genes related to MD217，and they were mainly involved in cell composition，biochemical processes，molecular functions and so on.They also referred to some processes in biological cells，such as catalysis and immunoreaction.The results of the Quantitative Realtime PCR showed that after the virus infection，MD217 upregulated the expression，and i...
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