猕猴桃灰霉病病原菌鉴定及室内药剂筛选
摘要通过病原菌形态特征、致病性测定及rDNAITS的同源性分析，确定江西奉新猕猴桃灰霉病的病原菌为Botrytis cinerea（有性态Botryotinia fuckeliana），该病菌在5~30 ℃的温度范围内均能正常生长，且该病菌只能通过伤口接种引起猕猴桃发病。采用菌丝生长速率法测定11种杀菌剂对该病原菌的毒力大小，结果表明，肟菌>戊唑醇水分散粒剂、氟硅唑乳油、异菌脲悬浮剂、苯醚甲环唑水分散粒剂、戊唑醇悬浮剂和吡唑醚菌酯乳油对其菌丝生长有较强的抑制作用，EC50值分别为0.118 0、0.288 6、0.355 2、1.541 8、1.760 2和1.778 4 μg/mL。
关键词猕猴桃灰霉病；Botrytis cinerea；毒力测定
中图分类号:S 436.634文献标识码：ADOI：10.3969/j.issn.05291542.2014.03.008Identification of the pathogenic fungus of kiwifruit gray
mold and indoor screening of fungicidesLi Cheng1，Jiang Junxi1，Zhao Shanggao2，Li Bangming2，Yu Qiang2，Tu Guiqing2（1.College of Agronomy，Jiangxi Agricultural University，Nanchang330045， China；
2. Agricultural Bureau of Fengxin County， Fengxin330700， China）AbstractThe pathogen causing gray mold on kiwifruit in Fengxin County of Jiangxi Province was identified as Botrytis cinerea（anamorph Botryotinia fuckeliana）by morphological characteristics, pathogenicity test and sequence homology analysis of rDNAITS. The pathogenic fungus could normally grow at 5-30 ℃, and penetrate fruit of kiwifruit only through wound infection. Following identification of the pathogen, toxicity test of 11 fungicides to B.cinerea was carried out in the laboratory by using mycelium growth rate method, and the results showed that trifloxystrobin>tebuconazole WG, flusilazole EC, rovral SC, difenoconazole WG, tebuconazole SC and pyraclostrobin EC had higher inhibition effects to the pathogen. The EC50 values were 0.118 0 μg/mL, 0.288 6 μg/mL, 0.355 2 μg/mL, 1.541 8 μg/mL, 1.760 2 μg/mL and 1.778 4 μg/mL, respectively.
Key wor...
|
== 试读已结束，如需继续阅读敬请充值会员 ==
|
|
本站文章均为原创投稿，仅供下载参考，付费用户可查看完整且有格式内容！
(费用标准：38元/2月，98元/2年，微信支付秒开通！) |
| 升级为会员即可查阅全文 。如需要查阅全文，请 免费注册 或 登录会员 |
|
