甘肃省马铃薯枯萎病(Fusarium,avenaceum)鉴定及其病原生物学特性

材料写作网    时间: 2020-08-06 04:30:56     阅读:次

摘要对甘肃省马铃薯枯萎病菌进行了分离、鉴定及培养特性的研究。结果表明，该病原菌大型分生孢子镰刀形，无色，多细胞，大小为（12.5~30）μm×（2.5~5）μm；小型分生孢子数量较多，无色，卵圆形或椭圆形，大小为（4.70~12.94）μm×（1.76~2.35）μm；ITS序列分析表明，病原菌与Fusarium avenaceum（JN6317483）亲缘关系最近，相似性达到100%，结合形态特征鉴定该病原菌为燕麦镰刀菌(Fusarium avenaceum)。该病原菌在5~40 ℃范围内均可生长，最适温度为20 ℃；供试的碳、氮源中，果糖和亮氨酸对该菌菌丝生长有显著的促进作用（P<0.05），而显著抑制菌丝生长的是氯醛糖和碳酸铵（P<0.05）；不同类型培养基中，燕麦片培养基对菌丝生长有显著促进作用（P<0.05）。

关键词马铃薯；枯萎病；鉴定；培养性状

中图分类号:S 435.32文献标识码：ADOI：10.3969/j.issn.05291542.2014.01.008Identification of potato wilt caused by Fusarium avenaceum

and the biological characteristics of its pathogenWang Yuqin，Yang Chengde，Chen Xiurong，Xue Li，Su Jianhong(College of Grassland, Gansu Agricultural University; Key Laboratory of Grassland Ecosystem,

Ministry of Education; Pratacultural Engineering Laboratory of Gansu Province; SinoU.S.Center for

Grazingland Ecosystem Sustainability, Lanzhou730070, China)AbstractThe pathogen causing potato wilt were isolated and identified by the rDNAITS sequences and biological characteristics in Gansu Province. The results showed that the macroconidia were hyaline, sickleshaped, multicellular, and the size was (12.5-30)μm × (2.5-5)μm. The microconidia were oval, and the size was (4.70-1294) μm×(1.76-2.35)μm. The rDNAITS sequence of the pathogenic fungus shared 100% similarity with that of Fusarium avenaceum (JN6317483). The morphologic characteristics and ITS sequence analysis all indicated that the pathogenic fungus was F.avenaceum. The suitable culture temperature was from 5 ℃ to 40 ℃, and the optimum was 20 ...

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