黄瓜绿斑驳花叶病毒磁免疫层析试纸条的研制
摘要用磁性纳米颗粒标记黄瓜绿斑驳花叶病毒的兔多克隆抗体建立了简便快速的磁免疫层析试纸条检测方法。该方法检测提纯病毒的灵敏度为0.1 μg/mL，检测葫芦病汁液可稀释10 000倍(重量／体积)。试纸条和黄瓜绿斑驳花叶病毒具有特异性反应，和同属的烟草花叶病毒、番茄花叶病毒、辣椒轻斑驳病毒和齿兰环斑病毒，未出现交叉反应。该试纸条可用于田间病害检测和诊断。
关键词黄瓜绿斑驳花叶病毒；磁免疫层析；试纸条
中图分类号:S 41文献标识码：ADOI：10.3969/j.issn.05291542.2014.03.019Magnetic immunochromatography assay for rapid detection of
Cucumber green mottle mosaic virusWei Meisheng1，Li Guifen1，Ma Jie1,Zhu Shuifang1,Wang Junping2,Wen Zhaohui2(1. Institute of Plant Quarantine, CAIQ, Beijing100029, China;
2. Gansu EntryExit Inspection and Quarantine Bureau, Lanzhou730020, China)AbstractRapid magnetic immunochromatography assay was developed by coupling magnetic nanoparticles with polyclonal rabbit antibody against Cucumber green mottle mosaic virus （CGMMV）. Sensitivity of the method was 0.1 μg/mL for detecting purified CGMMV. CGMMV could be detected in the extract of the infected Lagenaria siceraria leaves at a dilution of 10-4(W/V). The immunostrip was specific to CGMMV, without cross reaction with other tobamoviruses such as Tobacco mosaic virus, Tomato mosaic virus, Pepper mild mottle virus and Odontoglossum ringspot virus. The immunostrip is an ideal screening tool for field inspection and disease diagnosis.
Key wordsCucumber green mottle mosaic virus；magnetic immunochromatography assay；immunostrip 黄瓜绿斑驳花叶病毒（Cucumber green mottle mosaic virus, CGMMV）为烟草花叶病毒属（Tobamovirus）成员，是全国农业植物检疫性有害生物和我国进境检疫性有害生物。CGMMV 主要危害葫芦科作物，引起植物叶片斑驳、系统性花叶等症状，造成产量和品质下降，对生产危害很大［1］。
该病毒分布于英国、以色列、西班牙、希腊、沙特阿拉伯、印度、...
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