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高温对韭菜根际微生物多样性的影响

材料写作网    时间: 2020-09-09 04:05:04     阅读:


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摘要:为明确高温对韭菜根际微生物多样性的影响,本研究通过对高温处理的韭菜根部及根际土壤中的微生物进行分离纯化,并对分离得到的真菌和细菌分别采用ITS和16S rDNA基因分析方法进行鉴定。结果表明,未分离出真菌,分离鉴定出20种细菌。其中,在无韭蛆组的韭菜根部和根际土壤中分别分离出14、12种细菌,有韭蛆组的韭菜根部和土壤中分别分离出14、13种细菌,以变形菌门(Proteobacteria)和厚壁菌门(Firmicutes)细菌为主,其余的细菌属于放线菌门(Actinobacteria)。温度越高,厚壁菌门及放线菌门细菌出现频率越高;高温处理(35~50℃)的韭菜根部和根际土壤中细菌种类均少于其对照组。

关键词:韭蛆;韭菜;高温;根际微生物;多样性

中图分类号:S477+.1 文献标识号:A 文章编号:1001-4942(2019)11-0081-06

Effects of High Temperature on Rhizosphere

Microbial Diversity of Chives

Zhou Xianhong1, Wang Yan2, Fan Xiaojie1, Gao Huanhuan3, Qin Dongyun1, Zhuang Qianying1, Yu Yi1

(1. Institute of Plant Protection, Shandong Academy of Agricultural Sciences/

Key Laboratory for Plant Virology of Shandong Province, Jinan 250100, China;

2. Agricultural Comprehensive Law Enforcement Unit of Rushan City, Rushan 264500, China;

3. Shandong Institute of Grape, Jinan 250100, China)

Abstract To understand the effect of high temperature on the diversity of rhizosphere microorganisms of chives, the microorganisms from the roots (partial bulbs and nearby roots) and soils under high temperature treatment were separated, purified and identified through ITS and 16S rDNA genetic analysis. The results showed that no fungi were isolated and 20 kinds of bacteria were identified. Fourteen and twelve bacterial species were identified from the chive roots...

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