HPLC法测定清咽利喉合剂中黄芩苷的含量
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【摘 要】 目的:建立HPLC法测定清咽利喉合剂中黄芩苷含量的方法。方法:采用十八烷基键合硅胶为固定相;乙腈-0.1%磷酸(32∶68)为流动相;流速1.0mL/min;检测波长326nm,柱温30℃。结果:黄芩苷在0.05785~0.8677μg范围内呈良好线性(r=0.99977);平均回收率为99.9%,RSD为0.62%。结论:本法快速准确,杂质分离完全,重现性好,可用于清咽利喉合剂中黄芩苷的含量测定。
【关键词】 HPLC;清咽利喉合剂;黄芩苷
【中图分类号】R284.1 【文献标志码】 A 【文章编号】1007-8517(2019)17-0028-03
Abstract:ObjectiveTo establish a HPLC method for the determination of baicalin in Qingyan Lihou Mixture.Methods The octadecyl bonded silica gel was used as the stationary phase; acetonitrile: 0.1% phosphoric acid (32∶68) was used as the mobile phase; the flow rate was 1.0 mL/min; the detection wavelength was 326 nm, and the column temperature was 30℃.Results Baicalin showed good linearity in the range of 0.05785~0.8677μg (r=0.99977); the average recovery was 99.9%, and the RSD was 0.62%.Conclusion This method is fast and accurate, complete separation of impurities and good reproducibility. It can be used to determine the content of baicalin in Qingyan Lihou Mixture.
Key words:HPLC; Qingyan Lihou Mixture; Baicalin
清咽利喉合剂是曲靖市第一人民医院研制的院内制剂。由黄芩、金荞麦、玄参、麦冬等组成,具有养阴、清咽、清热解毒的功效,临床用于咽喉肿痛、急性扁桃体炎、咽颊炎、上呼吸道感染等症。其中黄芩为制剂中的君药,有着悠久的应用历史,始载于《神农本草经》,为唇形科植物黄芩Scutellaria baicalensis Georgi的干燥根,具有清热燥湿、泻火解毒、止血、安胎的功效[1]。《本草正》中记载:'枯者清上焦之火,消痰利气,定喘咳··,解瘟疫,清咽,疗肺萎··“,临床上主要用于肺热咳嗽、痈肿疮毒,咽喉肿痛等症。黄芩...
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