人参灰霉菌和人参黑斑菌对人参总皂苷的化学趋向性响应研究
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[摘要]采用平板法和孢子悬滴萌发法分别测定了人参灰霉菌和人参黑斑菌在3种趋化参数(浓度、温度和pH)下对人参总皂苷的化学趋向性响应。结果表明,人参灰霉菌在20 ℃和pH 6的培养条件下,对20 mg>L-1的人参总皂苷表现出较强的化学趋向性,趋化移动指数(CMI)为1293 0,趋化生长速率(CGR)为0476 0,孢子萌发率(SGR)为53%,菌丝干重(DWM)为0452 6 g>L-1;而人参黑斑菌则在25 ℃和pH 6的培养条件下,对2 mg>L-1的人参总皂苷表现出较强的化学趋向性,趋化移动指数(CMI)为1235 4,趋化生长速率(CGR)为0537 0,孢子萌发率(SGR)为67%,菌丝干重(DWM)为0494 8 g>L-1。显示人参灰霉菌和人参黑斑菌分别对低(2 mg>L-1)、中(20 mg>L-1)浓度的人参总皂苷具有正向的化学趋向性响应,而且受此趋化影响下的孢子萌发率、菌丝生长速率及菌丝生长量都得到了显著提高,但随皂苷浓度的升高而降低。
[关键词]人参灰霉菌;人参人参黑斑菌;人参总皂苷;化学趋向性
[Abstract]In this paper, three kinds of chemotactic parameters (concentration, temperature and pH) were determined by plate assay and spore germination method to research the chemotactic response of Botrytis cinerea and Alternaria panax, and their spores on total ginsenosides The results showed that Botrytis cinerea had strong chemotactic response at the midconcentration of total ginsenosides (cultivation temperature was 20 ℃ and pH value was 6), and the data of chemotactic migration index (CMI) was 1293 0, chemotactic growth rate (CGR) was 0476 0, spore germination rate (SGR) was 53%, and dry weight of mycelial (DWM) was 0452 6 g>L-1; however, Alternaria panax had strong chemotactic response at the lowconcentration of total ginsenosides (cultivation temperature was 25 ℃ and pH value was 6), and the data of chem...
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