柿果实水溶性提取物的抗氧化活性研究
摘要：水果具有加快人体体能恢复、延缓衰老的作用。研究利用DPPH法测定罗田甜柿（Diospyros kaki）果实水溶性提取物的抗氧化能力，经优化的最佳反应条件为40 ℃反应15 min。1 g/mL的柿果实水溶性提取物相当于1.5 mg/mL维生素C的抗氧化效率。对比甜柿果实提取物3种水性溶剂的抗氧化效率，水提取物的抗氧化活性最高，Tris-HCl缓冲液提取物次之，PBS缓冲液提取物抗氧化活性相对较差。
关键词：罗田甜柿（Diospyros kaki）；水溶性提取物；DPPH法；抗氧化活性
中图分类号：S665.2；R284.2 文献标识码：A 文章编号：0439-8114（2017）03-0542-03
DOI：10.14088/j.cnki.issn0439-8114.2017.03.038
Study on the Antioxidative Activity of Water-Soluble Extracts from Persimmon Fruit
WANG Shu-zhen，ZHENG Zhuang，ZHANG Zhi-wei，ZHANG Ming-ju，LI Zhi-liang，XIANG Fu，YANG Gu-liang
（Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization/Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains/College of Life Science，Huanggang Normal University，Huanggang 438000，Hubei，China）
Abstract： Fruits play an important part in quickening energy recovery and slowing aging process. the antioxidant capacity of water-soluble extracts from fruits of Diospyros kaki was measured with DPPH method. Results showed that the optimal reaction condition was at 40 ℃ for 15 minutes. The antioxidative capacity of 1 g/mL water-soluble extracts from persimmon fruit was equal to that of 1.5 mg/mL vitamin C. Comparing the antioxidative capacity of H2O，PBS，and Tris-HCl aqueous solvents，the best one was H2O，while the least one was PBS.
Key words： Diospyros kaki； soluble extracts； DPPH method； antioxidative activity
自由基是生物机体氧化反应过程中产生的游离态有害化合物，其氧化活性能够引起动脉粥样硬化、心血管疾病、肿瘤等多种慢性疾病[1]。天然抗氧化...
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