小麦黄花叶病毒病不同发生度土壤的微生物多样性研究
摘要：2016年于山东省临沂市、枣庄市、济宁市采集3组小麦黄花叶病毒病发生严重度不同地块的土壤，采用平板稀释分离法分别分离其中的真菌、细菌和放线菌，统计土壤微生物总量及3种可培养微生物的种类和数量，研究其与小麦病毒病发生程度的关系。结果表明，同一地块中细菌数量占绝对优势，其次为放线菌，真菌最少；但是分离得到的可培养真菌的种类和数量最多，其次为细菌，放线菌数量最少；并且发病严重地块的土壤微生物总量均高于轻病地块，其中发病严重地块的真菌数量和种类增加的最为显著，其次为细菌，放线菌基本无变化。
关键词：小麦黄花叶病毒病；土壤微生物；真菌；细菌；放线菌；多样性
中图分类号：S154.3文献标识号：A文章编号：1001-4942（2017）12-0044-06
Abstract Three groups of wheat soil on that different degree of wheat yellow mosaic virus occurred were collected from Linyi，Zaozhuang and Jining in 2016. The fungi， bacteria， actinomycetes were isolated by plate dilution separation method.The total number of soil microorganisms， and the types and numbers of three cultured microbes were counted， which were used to study their relationships with the occurrence degree of wheat yellow mosaic virus. The results indicated that bacteria assumed absolute superiority in number， followed by actinomycetes， and fungi was the least in the same places.But the type and number of cultured fungi were the largest， followed by bacteria， and those of actinomycetes were the least. The total number of soil microorganisms of serious disease soil was higher than that of light disease soil； the kinds and number of fungi in the serious disease soil had the most significant increasing， followed by bacteria， and actinomycetes had almost no change.
Keywords Wheat yellow mosaic virus；Total number of soil microorganisms；Fungi；Bacteria； Actinomycetes；Diversity
病毒病是小麦生产上一类重要的病害，近年来有逐渐加重的趋势。我国发现...
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