西兰花提取物萝卜硫素抑制肺癌细胞的生长和侵袭
【摘 要】萝卜硫素是从十字花科蔬菜中提取的多功能物质，研究已证实其具有抗癌、抗氧化等功效。肺癌是恶性程度高、具有转移倾向的恶性肿瘤，萝卜硫素抗肺癌的机制尚不是十分清楚。本研究通过CCK-8和transwell侵袭实验分析初步判断萝卜硫素对A549肺癌细胞活性和转移侵袭的影响，计算体外干预A549的IC50， 流式细胞学分析IC50浓度萝卜硫素对细胞周期和凋亡的影响，电泳分析DNA片段化改变。结果显示A549细胞活性对萝卜硫素剂量依赖性下降，萝卜硫素作用于A549 细胞的IC50 为3μg/mL， 该浓度的萝卜硫素抑制了A549细胞的转移侵袭，阻滞细胞于G2/M期，诱导细胞凋亡并使DNA发生片段化变化，下调TGF-β和NF-κB的表达。结果提示萝卜硫素可以抑制肺癌细胞的生长和侵袭，抑制炎症蛋白的表达，是肺癌辅助治疗的新途径。
【关键词】萝卜硫素；肺癌；生长抑制；凋亡
【中图分类号】R426 【文献标识码】A 【文章编号】1004-7484（2014）07-4589-02
【Abstract】Sulforaphane was a multifunction compound derived from brassicaceous vegetable such as broccoli， reports showed that Sulforaphane provided with effection of antitumor and antioxidant. Lung cancer is an aggressive malignancy with a tendency of early distant metastases， the antitumor function of sulforaphane was corroborated by numerous lines of evidence， but the anticancer mechanism of this compound has not been well obsvered. In this work， we analyzed vitality and invasion of A549 cells treated with sulforaphane by cell counting kit （CCK8） and transwell， then measure the half maximal （50%） inhibitory concentration （IC50） of sulforaphane for A549 cells. The cells cycle， apoptosis and DNA fragment were analyzed using Flow Cytometry Analysis and agarose electrophoresis， TGF-βand NF-κB were analyzed by western blot after treatment with 3μg/mL sulforaphane. Results showed that A549 cells proliferate and invade were inhibited by sulforaphane with a dose-dependent manner， IC50 of sulforaphane was 3μg/mL， and...
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