PCR—核酸试纸条法快速检测鸽子源性成分
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摘要 [目的]提高动物源性成分检测效率，建立PCR-核酸试纸条快速检测技术体系。[方法]针对物种特异性DNA序列，设计并标记引物；建立并优化PCR反应体系；利用通用核酸试纸条进行结果判读。[结果]试验表明，PCR-核酸试纸条特异性高；绝对灵敏度为0.001 3ng/μL，混合样品，实际灵敏度为0.01%；加工处理样品，实际灵敏度为0.1%；最低检测限检出率为100%，稳定性良好。[结论]综上，PCR-核酸试纸条方法是一种既有PCR反应的高灵敏度，又具有免疫学检测的特异性好的特点，同时操作简便的快速检测方法。
关键词 核酸试纸条；动物源；食品安全
中图分类号 TS207文献标识码 A文章编号 0517-6611（2016）20-083-03
Abstract [Objective] In order to improve the efficiency of detecting ingredients of animal origin， the PCR - nucleic acid strip rapid detection technology system was established. [Method] In view of the species specific DNA sequences， primers were designed and marked； the PCR reaction system was established and optimized； the results were interpretated by the nucleic acid strip. [Result] The results showed that： the PCR- nucleic acid strip is high specificity； absolute sensitivity is 0.001 3 ng/μL； the actual sensitivity in mixed samples is 0.01%； the actual sensitivity in processing sample is 0.1%； the detection rate of minimum detection limit is 100%， the stability is good. [Conclusion] To sum up， the high sensitivity as PCR and high specfticity as immunology made the PCR- chromatography strip valuable for the rapid detection method.
Key words Nucleic acid test paper； Animal origin； Food safety
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