CleistanthinA对黑素瘤细胞迁移及β—catenin蛋白的影响
【摘 要】
目的：研究Cleistanthin A（CA）對黑素瘤细胞迁移及β-catenin蛋白的影响。方法：通过划痕实验观察CA对A375细胞迁移活性的影响；通过Western blot实验观察CA对A375细胞中β-catenin蛋白的影响。结果：划痕实验显示：CA对A375细胞的迁移有抑制作用，且有剂量依赖性，且0.1μM这个浓度接近半数迁移抑制率；结合我们前期的实验得知：CA对A375细胞的生长活性有抑制作用，且有剂量依赖性，但低浓度的CA对A375细胞的活性抑制作用与阴性对照组相比虽有统计学意义却不显著[1]，因此选用0.03μM、0.1μM、0.3μM较低浓度组CA进行后面的实验，以降低细胞凋亡对迁移的影响。Western blot实验显示：CA对A375细胞中β-catenin蛋白表达有抑制作用，且有剂量依赖性，0.3μM浓度时与阴性对照组相比有统计学意义。结论 CA可抑制A375细胞的生长活性及迁移且可下调与生长及迁移密切相关的β-catenin蛋白的表达。
【关键词】 CleistanthinA，恶性黑素瘤，迁移，β-catenin
【中图分类号】R715 【文献标志码】
B 【文章编号】1005-0019（2018）16-230-01
Affect of Cleistanthin A on the migration and β-catenin in melanoma cell of A375
Pansheng，gaoteng，limeng
The skin Department，The Seventh People"s Hospital ， Changzhou Jiangsu，213003
Abstract Objective：to investigate the effect of Cleistanthin A（CA）treatment on melanoma cell migration and β-catenin. Methods The inhibitory activities of CA on cultured A375 cell migration were detected by Wound-healing assay；Western blot were used to detect the activity of β-catenin. Results Wound-healing assay shows：CA has obvious inhibitory effect to A375 cell migration activity， and in a dose dependent manner， and the concentration of 0.1μM is near the median inhibition rate. Combined with our previous experiments， we learned that： Cell viability is affected significantly by CA. It appears in a dosage manner. But the viability of A375 is not obviously affected by CA in low concentration， So the concentrations of 0....
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