利用GBS技术开发烟草SNP标记及遗传多样性分析
摘 要：研究烟草种质资源的遗传背景，为烟草种质资源的高效利用提供依据。采用GBS（genotyping-by-sequencing）技术，挖掘92份烟草种质资源的SNP位点，并进行遗传多样性和遗传结构分析。结果表明，测序共获得了147.165 Gb数据，平均每个样本1.599 Gb，筛选后共获得93 685个高质量的SNP位点；不同地理来源烟草群体等位基因数（Na）为1.27～1.93，观测杂合度（Ho）变化范围为0.22～0.76，期望杂合度（He）为0.32～0.59，多态性位点数（PLN）范围为15 877～44 249，多态性位点比率（PPL）为26.61%～74.17%，遗传多样性指数（H）为0.19～0.52，遗传距离（GD）为?0.0148～0.3849。基于遗传距离的NJ聚类将材料划分为两个类群；群体遗传结构分析表明，本研究所用材料基于模型可划分为4个亚群。通过GBS技术得到的测序数据量较大且质量较高，群体多态性较高，整体遗传多样性偏低，群体结构划分与种质来源不完全相关。
关键词：烟草；GBS；SNP；遗传多样性；遗传结构
中图分类号：S572.03 文章编号：1007-5119（2018）05-0017-08 DOI：10.13496/j.issn.1007-5119.2018.05.003
Abstract： To understand the diversity and genetic structure of tobacco germplasm resources and provide an important theoretical foundation for resource use， GBS （genotyping-by-sequencing） technology was used to discover SNP loci for 92 tobacco germplasm resources. With the SNPs genotyping data， the genetic diversity and genetic structure were analyzed. By using GBS， a total of 147.165 Gb of sequences was generated from the 92 tobacco germplasms， and each sample produced 1.599 622 Gb in average. After being screened， a total of 93 685 of high-quality SNP sites was retained. The range of number of alleles （Na） for different geographic sources of tobacco population was 1.27-1.93. The observed heterozygosity （Ho） was 0.22～0.76. The expected heterozygosity （He） was 0.32-0.59. The number of polymorphic loci was 15 877-44 249. The percentage of polymorphic loci （PPL） was 26.61%-74.17%. The genetic diversi...
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