基于LCMS代谢组学的雷公藤多苷致肝毒性生物标志物的初步筛查
打开文本图片集
[摘要]筛查雷公藤多苷片肝毒性产生的特征性生物标志物，并初步探讨其可能的作用机制，为雷公藤多苷片肝毒性提供可能的辅助检测指标和筛查诊断的方法。采用液相色谱质谱方法测定空白组大鼠与口服雷公藤多苷片高剂量模型组大鼠代谢指纹谱，利用多元统计分析方法比较2组的代谢谱差异，筛选出可能的潜在生物标志物。实验共筛选出20个在模型组中与正常组中差异性较大的代谢物（VIP>10），分析鉴定了7个代谢物，分别为6磷酸葡萄糖胺、溶血磷脂、色氨酸、胍基乙酸、3吲哚丙酸、可的松和泛醌，其代谢水平的变化表明氨基酸代谢、糖代谢、磷脂代谢和激素代谢等途径发生紊乱。由此推测雷公藤多苷片致肝损伤可能与三羧酸循环中能量代谢和尿素循环中氨基酸代谢，以及糖代谢异常有关，该研究可为今后进一步研究雷公藤肝毒性提供一定的数据支持。
[关键词]雷公藤多苷； 肝毒性； 代谢组学； 代谢物分析
[Abstract]In this paper， biomarkers of liver toxicity of Triptergium wilfordii based on metabolomics was screened，and mechanism of liver toxicity was explored to provide a reference for the clinical diagnosis for liver toxicity of Triptergium wilfordiiMS method was carried on the analysis to metabolic fingerprint spectrum between treatment group and control group The potential biomarkers were compared and screened using the multivariate statistical methods As well， metabolic pathway would be detailed description Combined with PCA and OPLSDA pattern recognition analysis， 20 metabolites were selected which showed large differences between model group and blank group（VIP>10） Seven possible endogenous biomarkers were analyzed and identified They were 6phosphate glucosamine， lysophospholipid， tryptophan， guanidine acetic acid， 3indole propionic acid， cortisone， and ubiquinone The level changes of above metabolites indic...
|
== 试读已结束，如需继续阅读敬请充值会员 ==
|
|
本站文章均为原创投稿，仅供下载参考，付费用户可查看完整且有格式内容！
(费用标准：38元/2月，98元/2年，微信支付秒开通！) |
| 升级为会员即可查阅全文 。如需要查阅全文，请 免费注册 或 登录会员 |
|
