利用IlluminaMiseq测序技术研究鲍内脏多肽对小鼠肠道微生物的影响
摘要[目的]研究鲍内脏活性物质对小鼠肠道微生物的影响。[方法]从鲍内脏中制备多肽并灌胃小鼠，利用Illumina Miseq测序技术分析小鼠肠道微生物的群落结构及动态变化。[结果]门水平上，小鼠肠道微生物最优势菌群为拟杆菌门（Bacteroidetes），占总序列的69.10%；其次为厚壁菌门（Firmicutes）和变形菌门（Proteobacteria），分别占总序列的23.50%和5.00%，变形菌门又以δ-变形菌纲居多。属水平上，最优势类群为拟杆菌目（Bacteroidales）的一个未知分类属，占30.33%；其次为拟普雷沃菌属（Alloprevotella）和拟杆菌属（Bacteroides），分别占12.47%和9.80%。灌胃鲍内脏多肽后，小鼠肠道中丰度前30位的菌属中有4个属的丰度上调，其中副拟杆菌属（Parabacteroides）的丰度显著上调。丰度下调的属有7个，其中毛螺菌科（Lachnospiraceae）一个未分类属和厌氧支原体属（Anaeroplasma）丰度显著下调。[结论]灌胃鲍内脏多肽后小鼠肠道微生物多样性呈增加的趋势，且在小鼠肠道中检测到一些与宿主健康相关的差异细菌类群。
关键词鲍内脏多肽；肠道微生物；Illumina Miseq测序
中图分类号S852.6文献标识码A
文章编号0517-6611（2019）08-0093-05
doi：10.3969/j.issn.0517-6611.2019.08.024
Abstract[Objective] To study the effects of visceral active substance of abalone on intestinal microbe of mice by Illumina Miseq sequencing. [Method] Polypeptide extracted from abalone viscera and mice was fed with it by intragastric administration. The community structure and dynamic changes of intestinal microbe of mice were analyzed by using Illumina Miseq sequencing. [Result] The most dominant phyla of bacteria in the intestine of mice were Bacteroides， accounting for 69.10% of the total sequence， followed by Firmicutes and Proteobacteria（ accounting for 23.50% and 5.00% of the total sequences， respectively）. Proteobacteria was dominated by δProteobacteria.At the genus level， the most dominant group was norank_f_Bacteroidales_S247_group， accounting for 30.33%， followed by Alloprevotella and Bacteroides（accounting for 12...
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