水溶性富勒烯C60的制备及其血液,相容性研究
（中国人民解放军军事医学科学院毒物药物研究所，北京100850）
[摘要]目的：制备γ环糊精-C60超分子化合物，改善C60的水溶性和血液相容性。方法：采用研磨法制备γ环糊精-C60超分子化合物，利用红外光谱对其结构进行表征，通过溶血实验及血浆复钙实验对C60、γ环糊精-C60的血液相容性进行评价。结果：红外光谱结果表明γ环糊精与C60发生包埋，γ环糊精-C60在双蒸水中放置24h无聚沉，样品浓度为512μg/mL 、102.4μg/mL 、25.6μg/mL时，C60的溶血率分别为13.75%±0.26%、6.02±0.12%、3.21±0.19%，γ环糊精-C60的溶血率分别为3.74%±0.39%、1.13%±0.12%、0.55%±0.19%；阳性对照、C60、γ环糊精-C60的复钙时间分别为10min 、15min、20min，对应吸光度值分别为1.09、0.68、0.51 。结论：经γ环糊精修饰后C60的水溶性和血液相容性得到改善。
[关键词]C60；γ环糊精；水溶性；血液相容性
中图分类号：O657文献标识码：A文章编号：2095-5200（2014）04-001-03
DOI：10.11876/mimt201404001
Preparation of water-soluble fullerene C60 and its hemocompatibility studyLIU Yan，SUN lan，YAO Hong-juan，ZHANG Ying-ge.（Institute of Pharmacology and Toxicology，Academy of Military Medical Sciences，Beijing 100850）
[Abstract]Objective：To improve the water-solubility of C60, γ cyclodextrin-C60 supramolecular compounds were prepared. Methods：γ cyclodextrin-C60 supramolecular compounds were prepared by grinding way. The structure of γ cyclodextrin-C60 was characterized by infrared spectroscopy. Then, through the hemolysis experiment and plasma recalcification experiment, the hemocompatibility of C60 and γ cyclodextrin-C60 have been evaluated. Results：FTIR results showed that the γ cyclodextrin embedded with C60. γ cyclodextrin-C60 placed 24h without coagulation in double distilled water. In the concentration of 512μg/mL, 102.4μg/mL, 25.6μg/mL the hemolysis rate of C60 were 13.75% ± 0.26%, 6.02 ± 0.12%, 3.21 ± 0.19% respectively, while γ cyclodextrin-C60 were 3.74% ± 0.39%, 1...
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