石蜡切片方法的改良
摘要[目的]快速且安全高效制作石蜡切片。[方法]采用不同的方法，对植物材料进行透明和脱蜡以及包埋处理，把材料制成透明的玻片标本，利用正丁醇代替二甲苯作为透明剂和脱蜡剂，其他和传统的石蜡切片方法相同。[结果]正丁醇既能与乙醇混合，又能与中性树胶相融合；正丁醇作用相对较缓和，透明后的组织平整，没有明显的收缩、变形现象，并且不存在透明时间过长的问题，还减少了对实验人员的伤害。[结论]该方法可以快速地制作石蜡切片，且安全有效。
关键词石蜡切片；透明剂；正丁醇；花
中图分类号S-03文献标识码
A文章编号0517-6611（2017）32-0006-03
Method Improvement of Paraffin Section
ZHAO Yalin，YAN Qingdi，FENG Jiajia，WANG Fengru*，DONG Jinggao* et al（College of Life Science，Agricultural University of Hebei/Key Laboratory of Plant Physiology and Molecular Pathology in Hebei Province，Baoding，Hebei 071001）
Abstract[Objective] The aim was to make paraffin section safely and fast.[Method] Different methods were used to transparent and dewax the plant material and embedding，and the material was made into a transparent glass slide，and the nbutanol could instead of xylene.The other steps were the same as the traditional paraffin section method.[Result] The nbutanol not only could be mixed with ethanol，but also could be mixed with neutral gum.The nbutanol was relatively gentle，could obtain transparent tissue after formation，had no obvious shrinkage，deformation and transparent problem of long time，and reduced the damage to experimental staff.[Conclusion] The method could make paraffin section safely and fast.
Key wordsParaffin section；Clearing agent；nbutanol；Flower
石蠟切片是植物或者动物组织学常规制片技术中最为广泛应用的方法。在传统的石蜡切片时，常采用二甲苯作透明剂和脱蜡剂。二甲苯的细胞学毒性主要体现在诱导线粒体解偶联和氧化应激反应[1-2]，石蜡切片不仅用于观察正常细胞组织的形态结构，也可以作为病理学和法医学等学科用...
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