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奶牛乳房炎主要病原菌的分离鉴定及耐药性研究

材料写作网    时间: 2020-09-23 04:04:50     阅读:


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摘要:为了解奶牛乳房炎病原菌的菌群分布及耐药性情况,对湖北省一奶牛场开展为期1年的病原菌分离鉴定和耐药性研究。结果表明,从165份乳样中共分离鉴定出9个科16个属的235株病原菌。其中葡萄球菌83株,占35.0%;中型无绿藻36株,占13.5%;鲁氏不动杆菌28株,占11.9%;大肠杆菌12株,占5.1%;停乳链球菌7株,占2.9%;其他病原菌占31.6%。耐药试验结果显示,20株分离菌对测试的14种抗菌药物均产生不同程度耐药性,最少对1种药物产生耐药性,最多达到14种。敏感性最高的抗菌药物为氟苯尼考,敏感率达到75%。研究结果可为奶牛乳房炎的防控和临床用药提供基础性数据。

关键词:奶牛乳房炎;病原菌;分离鉴定;耐药性

中图分类号:S857.2+6 文献标识码:A

文章编号:0439-8114(2019)21-0145-05

DOI:10.14088/j.cnki.issn0439-8114.2019.21.031

Abstract: In order to understand the microflora distribution and drug resistance of the pathogenic bacteria of cow mastitis, a one-year pathogen isolation and identification and drug resistance study was conducted in a dairy farm in Hubei Province. The results showed that 235 strains of pathogens belonging to16 genera of 9 families were isolated from 165 milk samples. Among them, 83 strains were Staphylococcus, accounting for 35%; 36 strains were Protothecazopfii, accounting for 13.5%; 28 strains were Acinetobacterrubrum, accounting for 11.9%; 12 strains were Escherichia coli, accounting for 5.1%; 7 strains were Streptococcusdysgalactiae, accounting for 2.9%; and other strains accounting for 31.6%. The results of drug resistance test showed that 20 isolates had different degrees of drug resistance to the 14 antibiotics tested. The pathogen is resistant to at least one drug, and the highest is resistant to 14 drugs. The mos...

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