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小麦腐霉根腐病原鉴定Ⅱ

材料写作网    时间: 2020-08-01 04:23:30     阅读:次     手机站

摘要:2015年冬小麦返青期,山东单县冬小麦出现叶片变黄、植株矮小,毛细根上出现开水烫过的水渍状,造成了部分死苗现象,点片发生。为明确病原和有效防治该病害,本研究对病原菌进行分离培养、致病性测定、形态学观察及分子生物学鉴定。结果表明,山东单县冬小麦根腐病(称为腐霉根腐病)由瓜果腐霉Pythium aphanidermatum引起。

关键词:腐霉根腐病;瓜果腐霉;冬小麦

中图分类号:S435.121.4+7文献标识号:A文章编号:1001-4942(2017)11-0095-03

Identification of Pathogen Causing Wheat Root RotⅡ

Zhang Yueli, Qi Junshan, Zhang Bo, Ma Liguo, Xu Zuoting, Li Changsong, Qi Kai

(Institute of Plant Protection, Shandong Academy of Agricultural Sciences/

Shandong Provincial Key Laboratory of Plant Virology, Jinan 250100, China)

AbstractA disease was observed on winter wheat in Shanxian of Shandong Province at the returning green stage in 2015. The infected wheat seedlings showed yellow leaves,dwarf plants and water-soaked capillary roots, and some seedlings died. The disease occurred in spots and patches. To identify the pathogen and control the disease effectively, the pathogenic bacterium was isolated from the infected wheat roots. Based on its morphology biological characteristics, pathogenicity and molecular biology identification, the pathogen was identified as Pythium aphanidermatum.

KeywordsPythium root rot; P. aphanidermatum; Winter wheat

小麦腐霉根腐病是世界性病害,现全国均有分布。小麦受到腐霉菌侵染后,根系常表现为变褐腐烂,地上部分生长不良,产量降低,种子和幼苗在出土前就可霉烂和死亡。近年来,山东菏泽单县冬小麦返青后出现了外围叶片萎蔫的症状,呈点片发生,发病轻的植株根系水渍状,似开水烫过,发病重的植株根部变褐腐烂,地上部分生长不良,造成了死苗现象。本研究通过病原菌分离、致病性测定、形态特征及分子鉴定,明确引起冬小麦根腐病(称其为小麦腐霉根腐病)的病原,以期为该病害发生规律的探究和综合防治措施的制定...



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