发酵虫草菌粉多糖免疫活性实验研究
摘要目的：探究发酵虫草菌粉多糖的免疫活性。方法：发酵虫草菌粉通过水提醇沉、复溶、透析、浓缩、冷冻干燥得多糖，命名为CSMP60，其提取条件：60 ℃水提、提取时间4 h、料液比1∶15、提取次数2次。以小鼠免疫细胞为材料，检测CSMP60的免疫活性。结果：CSMP60能促进脾细胞的增殖（P<005），明显促进小鼠腹腔巨噬细胞分泌TNFα（P<005），有促进脾淋巴细胞分泌IFNγ的趋势。结论：CSMP60可能是发酵虫草菌粉产生免疫活性的效应物质之一。
关键词发酵虫草菌粉；多糖；细胞增殖；脾细胞；巨噬细胞；免疫活性；肿瘤坏死因子；γ干扰素
Experimental Study on Immune Activities of Polysaccharides from Fermented Cordycepssinensis Powders
Jiang Hongliang1，Zhao Xiunan2，Ma Hao2，He Lifang3，Shan Junjie2，Wang Hainan1，4
（1 School of Pharmaceutical Sciences，Guizhou University，Guiyang 550025，China； 2 Institute of Military Medicine，Academy of Military Sciences，Beijing 100850，China； 3 Hunan University of Chinese Medicine，Changsha 410208，China； 4 China Food and Drug Administration，Beijing 100053，China）
AbstractObjective：To investigate the immunological activity of polysaccharides from fermented Cordycepssinensis powders.Methods：Polysaccharides from fermented Cordycepssinensis powders were obtained through water extraction and alcohol precipitation，reconstitution，dialysis，concentration and freezedrying，named after CSMP60.Its extraction conditions：60 ℃ water extraction，extraction time 4h，solidliquid ratio 1∶15，the number of extractions 2 times.The immune activity of CSMP60 was measured using mouse immune cells.Results：CSMP60 could promote the proliferation of spleen cells （P<005），and significantly promote the secretion of TNFα from mouse peritoneal macrophages （P<005）.It has a tendency to promote the secretion of IFNγ by spleen cells.Conclusion：CSMP60 may be one of the immunocompetence substances of fermented Cordycep...
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