Effect,of,electroacupuncture,at,pericardium,meridian,on,D-ser,and,NMDAR,in,acute,phase,of,MCAO,rats
YUAN Liu-mei, XIA Yun, LU Xiao-ye, LIU Lei, ZHOU Ying, LOU Bi-dan
1.Hunan University of Traditional Chinese Medicine, Changsha 410007, China
2.The First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, China
3.Guangdong Nanhua Vocational College of Industry and Commerce, Guangzhou 510507, China
4.Changsha Health Vocational College, Changsha 410100, China
Keywords:
ABSTRACT
Acute ischemic stroke (AIS) is the most common type of stroke in China [1], and its pathogenesis and treatment methods have been the focus of research.Excitatory toxicity mediated by N-methyl-Daspartic acid receptor (NMDAR) is a hot topic in stroke research.D-serine (D-Ser), as the main endogenous ligand of the " glycine site " on the NR1 subunit of NMDAR, can regulate the function of NMDAR[2].Based on the theory of heart and brain governing spirit[3], the previous study of our research team[4-7]confirmed that electroacupuncture at pericardium meridian can improve the symptoms of neurological deficits in MCAO rats, the therapeutic mechanism is closely related to the regulation of D-Ser synthesis and release, regulating the expression of NR1, NR2A, NR2B, and affecting intracellular Ca2+content.On the basis of previous studies,this experiment further explored the effect of electroacupuncture on D-ser content and NMDAR expression in brain tissue of MCAO rats in the acute phase by adding exogenous D-Ser reagent.
2.1 Experimental animals and grouping
Thirty-six SPF healthy adult male SD rats, weighing 250-300 g, 3-4 months old, were purchased from Hunan Slake Jingda Experimental Animal Co., Ltd.They were randomly divided into the following 6 groups : nor...
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